Gel filtartion chromatography
also known as size exclusion chromatography or molecular sieve chromatography, separates target molecules by selecting peaks in descending order based on their size.
Gel filtration chromatography is often used for subsequent purification stages with low impurities. It is also
used for purification of smaller samples.
It can also be used in the crude purification stage for group separations (such as desalting).
Gel filtration chromatography requires only a single buffer; the type of buffer has little impact on the separation. Adding 150mM sodium chloride to the buffer can effectively reduce nonspecific adsorption of the target protein.
Gel filtration media separation range (globulin Da)
Gel filtration media are designed for the separation and purification of biomolecules based on molecular size. They provide an efficient range for globulin and other proteins, ensuring high resolution, reproducibility, and gentle processing of sensitive biological samples.
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Application Case
1. Separation of Substances of Different Molecular Weights by Focurose 4FF/6FF
2. Comparison of Focurose 4FF purified influenza vaccine and imported brandsrands
List of agarose gel filtration media
Product name | Separation range (globulin) | Particle size range µm | Maximum flow rate cm/h | pH stability long term [short term] | Application/Features |
Focurose 4B |
| 45-165 | 11 | 4-9 [4-9] | Determination of molecular weight of proteins, polysaccharides and peptides |
Focurosis 4FF |
| 45-165 | 250 | 2-12 [2-14] | Separation of biomacromolecules such as vaccines and viruses |
Focurose 6FF |
| 45-165 | 300 | 2-12 [2-14] | Separation of biological macromolecules such as plasmid DNA, viral vaccines, etc. |
Focurose 200PG |
| 25-45 | 90 | 3-12 [1-14] | Separation and purification of monoclonal antibodies and proteins |
Focurose 75PG |
| 25-45 | 90 | 3-12 [1-14] | Separation and purification of peptides and low molecular weight proteins |
Focurose G25+ |
| 45-165 | 300 | 3-12 [1-14] | Biomolecule desalting, buffer exchange |
