Flow cytometry (FCM) is a high-speed, multi-parameter quantitative analysis technique used to study individual cells and biological particles at both the cellular and molecular levels.
Using optical detection and laser-based measurement, this powerful method enables researchers to count, identify, and classify single cells or particles suspended in a flowing liquid stream.
Flow cytometry can analyze tens of thousands of cells per second, capturing multiple biological parameters from each individual cell.
Thanks to its speed, precision, and analytical accuracy, FCM has become one of the most advanced and reliable tools in quantitative cell analysis, widely applied in immunology, oncology, hematology, and molecular biology research.
Magnetic Fluorescent Microspheres
Flow cytometry is an advanced high-throughput detection technology that utilizes a flow cytometer as the main detection platform. It employs microspheres of varying particle sizes, each carrying fluorescent signals of different intensities, as solid-phase carriers for multi-parameter analysis.
This technology enables simultaneous detection and quantification of multiple analytes in a single sample, offering high throughput, wide dynamic range, excellent precision, and strong reproducibility.
Flow cytometry is extensively used in clinical diagnostics and biomedical research for diseases such as cancer, autoimmune disorders, cardiovascular conditions, inflammatory diseases, and infections.
It provides valuable insights for differential diagnosis, treatment monitoring, and disease evaluation.
9-Fold Magnetic Fluorescent-Encoded Microspheres
No Brand magnetic fluorescent microspheres are available in 4 μm and 5 μm particle sizes and are capable of simultaneous detection of up to 18 parameters.
These microspheres feature uniform particle size distribution, stable fluorescence, and superparamagnetic properties, making them ideal for use on automated flow cytometry platforms and high-throughput testing systems.
Product Advantages
Strong and stable fluorescence intensity, with low intra-peak CV values for high detection precision
Rich surface functional groups allow efficient antibody or ligand coupling, enhancing assay sensitivity
Multiplex detection capability enables the simultaneous measurement of multiple targets in one sample
Uniform particle size and superparamagnetic properties, compatible with automated testing and magnetic separation systems
Technical Specifications
| Parameter | Specification |
| Material | Fe₃O₄ / PS |
| Fluorescence channel | APC |
| Particle size | 4 μm, 5 μm |
| Multiplex detection | 9 single-color fluorescence |
| Particle count | 1.2 × 10⁸ particles/mL |
| Surface group | Carboxyl (-COOH) |
Performance Verification
Applications
Multiplex flow cytometry assays
Immunoassay and biomarker detection
Disease research and diagnostic screening
Automated magnetic separation and liquid handling systems
High-throughput quantitative analysis
Product name | Concentration | Fluorescent Intensity | Size | Price | ||
Magnetic Fluorescence-encoded Microspheres | 5.0μm | 1.2x108Count/mL | ~800W | 1mL，5mL，10mL | ||
Magnetic Fluorescence-encoded Microspheres | 5.0μm | 1.2x108 Count/mL | ~400W | 1mL，5mL，10mL | ||
Magnetic Fluorescence-encoded Microspheres | 5.0μm | 1.2x108 Count/mL | ~200W | 1mL，5mL，10mL | ||
Magnetic Fluorescence-encoded Microspheres | 5.0μm | 1.2x108 Count/mL | ~100W | 1mL，5mL，10mL | ||
Magnetic Fluorescence-encoded Microspheres | 5.0μm | 1.2x108Count/mL | ~50W | 1mL，5mL，10mL | ||
Magnetic Fluorescence-encoded Microspheres | 5.0μm | 1.2x108 Count/mL | ~25W | 1mL，5mL，10mL | ||
Magnetic Fluorescence-encoded Microspheres | 5.0μm | 1.2x108 Count/mL | ~10W | 1mL，5mL，10mL | ||
Magnetic Fluorescence-encoded Microspheres | 5.0μm | 1.2x108 Count/mL | ~5W | 1mL，5mL，10mL | ||
Magnetic Fluorescence-encoded Microspheres | 5.0μm | 1.2x108 Count/mL | ~2W | 1mL，5mL，10mL |
Absolute Counting Microspheres
This series of absolute counting microspheres is specially developed for the precise quantification of cells or biological particles.
Designed for use with flow cytometers and automated cell counters, these microspheres allow accurate determination of the absolute number of cells or particles by comparing the ratio between the target cells and the microspheres in the same sample.
The product line includes both non-fluorescent (FC) and fluorescent (FM) microsphere options:
Non-fluorescent absolute counting microspheres (FC): blank carboxylated polystyrene beads ideal for single-cell or particle counting.
Fluorescent absolute counting microspheres (FM): carboxylated polystyrene beads embedded with fluorescent dyes (FITC, PE, APC), suitable for multiparameter detection, cell classification, and absolute quantification in flow cytometry.
Product Advantages
Highly uniform particle size and fluorescence intensity ensure superior counting accuracy and reproducibility
Single-platform absolute counting method provides consistent and reliable quantitative results
Easy-to-use formulation suitable for a wide variety of cell types and biological particle analyses
Multiple fluorescence options (FITC, PE, APC) enable flexible multiplex analysis
Technical Specifications
| Parameter | Specification |
| Material | Polystyrene microspheres / Polystyrene microspheres with fluorescent dyes |
| Concentration | 2 × 10⁷ particles/mL |
| Density | 1.05 g/cm³ |
| Fluorescence channel | Three-color fluorescence (FITC, PE, APC) / Two-color fluorescence (FITC, PE) |
| Uniformity | CV < 5% |
| Dispersion medium | Pure water |
| Additives | Trace surfactants and preservatives |
| Storage conditions | 2–25 °C (Do not freeze) |
Applications
Flow cytometry-based absolute cell counting
Quantification of blood cells, immune cells, and microorganisms
Calibration and validation of cytometers and particle analyzers