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AccuNanoBead™ Magnetic Beads for Nucleic Acid Isolation and Molecular Biology Applications


Making the Best of a Rapid Solution for Biomolecule Purification

AccuNanoBead™ is a next-generation magnetic nanoparticle solution designed for high-performance DNA, RNA, and protein purification. Engineered with superparamagnetic properties, these beads offer rapid, efficient, and reproducible isolation of nucleic acids and biomolecules, making them ideal for PCR, NGS, proteomics, and other molecular biology applications. Compatible with automated platforms and manual workflows, AccuNanoBead™ ensures maximum yield, minimal sample loss, and exceptional purity, helping researchers achieve reliable and reproducible results in every experiment.

Products Description
Recently, many of bio related institutes and industries are
approaching their research applying magnetic beads.
Magnetic beads have been used widely in purification of
biosimilar proteins and antibody drugs. Magnetic beads have
been developed to overcome weakness of existing resin and
to automate purification process. The principle of magnetic
beads are coating with appropriate functional group in the
surface of magnetic beads. With the functional group,
magnetic beads can combine protein and then using external
magnetic field, Magnetic beads is moved for isolation.

Features of Magnetic Beads


● Sufficient Surface Area-to-Volume Ratio for Effective Purification, Spherical and Magnetic, Nano-sized Silica Beads
● Suitable for Separating Target Biomolecules

● Superior Purification Method in terms of Speed, Accuracy and Cost

Rapid Experiment as Result of Fast Binding and Easy Handling
●Notably Efficient Purification Yield due to Large Surface Area
●High Quality Results by Reducing Nonspecific Binding

●Easily Applicable to Automated System


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Specifications of Silica Magnetic Beads 


● Matrix : silica-coated Fe3O4
● Average particle size : 100 nm, 200 nm, 400 nm
● Working temperature : 0~100℃
● Storage solution : D.W.
● Storage temperature : Room Temperature

● pH stability 

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Applications of Silica Magnetic Beads

● Nucleic Acid Purification
● Total RNA Purification
● Viral DNA and RNA Purification
● Cell Purification
● mRNA Purification
● Peptide Preparation

● Antibody Purification


FE-SEM and TEM Image of Magnetic Beads 


Figure 1. FE-SEM photograph shows Bioneer’s 400 nm, 200 nm, 100 nm Magnetic Nanobeads are uniform spheres. 

Comparison of Bead Appearance 


Figure 2. SEM photographs of Magnetic Beads from: (a) Bioneer; (b) company A; (c) company B; (d) company C; (e) company D.

The photographs above show Bioneer’s Magnetic Beads are uniform spheres that are much smaller in size than others.

Simpler, Faster and More Effective Purification

Eliminate Repeated                  
Pipetting or

Centrifugation

       

Figure 3. Schematic Purification Process.
Traditional purification method using agarose beads needed centrifugation and repeatated pipetting.
For this reason, purification procedure is complicated and lose of samples is unavoidable during each step.
AccuNanoBead™ enable user to remove supernatant easily without lose of samples, because it can be
immobilized by magnet.

Complete Recovery
within 1 Second

  

Figure 4. Recovery test of Magnetic Beads from AccuNanoBead™ (Dark Blue) and Competitor L (Brown).
Recovery of AccuNanoBead ™ is completed within 1 second. It is 15 times faster than Competitor L product.

Rapid recovery allows user to finish experiment quickly, thus save time.

Acquire Increased
Purification Yield
 

Figure 5. Comparison of purification yield.
Purification yield with AccuNanoBead™ is notably higher than competitors’ products.
This is due to increased surface area of Bioneer’s beads in accordance with a smaller particle size.
Purification yield is amount of purified target protein from loading protein (Ni-NTA magnetic beads).

Products of AccuNano Bead ™

AccuNano Bead ™ Silica Coated Magnetic Nanobeads AccuNanoBead Plasmid DNA Purification Using Magnetic Nanobeads

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Figure 6. Silica coupling reaction.

 Plasmid DNA was purified using  AccuNanoBead ™ Silica Magnetic AccuNanoBead  Beads and  Maglisto™ Magnetic Separation  Rack through the MagListo process of cell lysis, DNA capture, impurity removal and finally DNA  elution without the use of centrifugation, and the result was compared with that from column prep.

AccuNanoBead ™ Amine Magnetic Nanobeads

Figure 7. Amine coupling reaction Bioneer AccuNanoBead™ NH2 Magnetic Nanobeads are product coated with Amine on the surface of silica magnetic nano
beads and are suitable for conjugation of large protein. The Beads can form covalent conjugation with epoxide- or carboxycontaining
ligands. The simple and efficient collection in magnetic fields, such as Maglisto™ Magnetic Separation Rack, allows
for easy washing and removal of supernatant after coupling.

AccuNanoBead ™ Carboxyl Magnetic Nanobeads

Figure 8. Carboxyl coupling reaction Bioneer AccuNanoBead™ COOH Magnetic Nanobeads are magnetic beads coated with high density of carboxyl functional
groups on the surface. The beads are used to covalently conjugate primary amine- containing ligands via a stable amide
bond. Bioneer AccuNanoBead™ COOH Magnetic Nanobeads are suitable for conjugation of Amine modified Oligo Nucleic Acid
(DNA & RNA) and large size of protein. The simple and efficient collection in magnetic fields, such as Maglisto™, allows for easy

washing and removal of supernatant after coupling

AccuNanoBead ™ Epoxy Magnetic Nanobeads

Figure 9. Epoxy-Coupling Reaction. AccuNanoBead Epoxy Magnetic Nanobeads are silica magnetic nano beads coated with high density epoxy
ligands on the surface. Beads can form covalent bond with hydroxyl-, amine and thiol- ligands. Coupling reaction
of each ligand is favored at pH 11-12, pH>9 and pH 7.508.5, respectively. Epoxy ligands is able to react with these ligands.
It can be applied to cell sorting, immunoprecipitation and etc.

AccuNanoBead ™ C18 Magnetic Nanobeads

Figure 11. Streptavidin & Biotin coupling reaction.
Bioneer AccuNanoBead™ streptavidin Magnetic Nanobeads are silica-based magnetic beads coated with high density
streptavidin on the surface. The Beads are designed, tested and quality controlled for use in immunoprecipitation,
co-immunoprecipitation, in-vitro diagnostic assay and magnetic bead cell sorting, rapid single-step capture of biotinylated
molecules such as DNA, RNA, antibody or protein from cell lysates or hybridization reactions.

Bioneer AccuNanoBead ™ Biotin Magnetic Nanobeads are densely coated with Biotin. Nanobeads are utilized in the magnetic
separation of avidin and streptavidin-labeled molecules. Biotin magnetic beads are stable, pre-blocked beads with high binding
capacity that provide rapid and efficient biomolecule purification from complex samples. Design of Bioneer AccuNanoBead™
Biotin Magnetic Nanobeads enables faster binding kinetics, High yield, purity, and quality in many biomedical and research

applications.


AccuNanoBead ™ Ni-NTA Magnetic Nanobeads

Figure 12. Ni-NTA magnetic bead binding reaction. 
Bioneer AccuNanoBead ™ Ni-NTA Magnetic Nanobeads is
designed for specific purification of 6x His-tagged proteins.
Ni-NTA Nanobeads have nitrilotriacetic acid (NTA) groups
with charged nickel covalently bind to surface of silica
nanobeads. Due to the high affinity, Ni-NTA beads can be
used for capturing 6x His-tagged proteins easily and
efficiently. Among them, Ni-Charged magnetic particles
coating physiological lipids have excellent compatibility
for in vivo studies. These highly binding beads are suitable

for a wide range of research.


AccuNanoBead ™ Thiol Magnetic Nanobeads

Figure 13. Thiol coupling reaction.
Bioneer AccuNanoBead™ Thiol Magnetic Nanobeads, silica-based magnetic beads coated with high density Thiol functional
groups on the surface. The beads are used to reversible couple thiol-containing ligands. Thiol-coated magnetic Beads are
recommended for conjugation of DNA, RNA, proteins, and specific cells. The beads are useful for small peptides because

the long-arm hydrophilic linker may reduce steric hindrance.

AccuNanoBead ™ Protein A,G,L Magnetic Nanobeads

Figure 14. Antibody Binding region of Protein A, G, L and coupling reaction scheme 

Bioneer AccuNanoBead™ Protein A, G, L Magnetic Nanobeads are product coated with recombinant Protein A, Protein G or
Protein L on the surface of silica magnetic nano beads. Protein A, G, L exhibits high affinity for subclasses of IgG from many
species (include Human, Mouse, Rat, Guinea Pig and Pig) and can be used for immunoprecipitation assays with these antiboies.
The beads are also widely used for efficient purification of IgG antibodies. But IgM cannot bind to Protein A and G because of
their structure. Only Protein L binds to IgM. Bioneer AccuNanoBeadTM Protein A, G, L Magnetic Nanobeads are suitable for

Antibody purification, Immunoprecipitation, Antigen-Antibody interaction studies and etc.