Thermo Fisher Scientific Demonstrates Superiority of Data-Dependent Decision

Thermo Fisher Scientific Inc., the world leader in serving science, today announced a new application note entitled “Enhancing Phosphotyrosine Proteome Coverage using a Combined ETD and CID Approach on a LTQ Orbitrap XL ETD”. The application note is a result of the cooperation with the Netherlands Proteomic Centre and in particular the
Biomolecular Mass Spectrometry and Proteomics Group at Utrecht University,headed by Albert Heck. It demonstrates how electron transfer dissociation (ETD) and collision induced dissociation (CID) are complementary fragmentation techniques, that when used together on the Thermo Scientific LTQ Orbitrap XL ETD mass spectrometer, provide significantly greater phosphoproteome coverage than mass spectrometry (MS) methods relying on CID alone. By using intelligent data-dependent decision tree (DDDT) logic to choose whether to perform ETD or CID in real-time, significantly more phosphopeptides can be identified, along with unambiguous determination of the phosphorylation sites.

Source: The Scott Partnership